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Flint's habit of "pre-flushing the tap" the night before samples were collected might be skewing things.
HUVECs or RAW264.7 were pretreated with 0 uM or 20 uM FG-4592 before samples were collected.
Before samples were collected for fatty acid and pigment analysis (see below), cultures were acclimated to the different temperatures and irradiance levels in semi-continuous batch mode for at least one week; fast growing cultures were diluted with fresh medium to avoid nutrient limitation.
Alternatively, some chemicals may have been consumed (in the case of beebread) before samples were collected.
Both written and oral consent was obtained before samples were collected.
Following the forced swim test animals were allowed 1 week to recover before samples were collected for gene expression profiling.
Similar(47)
Cells were incubated for 24 h before medium samples were collected for cytokine measurements and for 4 h or 24 h in case of intracellular protein analyses.
In order to collect fresh and representative samples, the boreholes were pumped on the average 5 min before the samples were collected.
Dialysis perfusion was started one hour later, at which time the mice were left undisturbed for approximately 2 h before baseline samples were collected.
Towards this aim, before ChIP samples were collected, a portion of yeast culture was treated with high concentration of bleomcyin (7.5 mU/ml) for two hours.
All symptomatic patients were treated before serum samples were collected.
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CEO of Professional Science Editing for Scientists @ prosciediting.com