Sentence examples for before sample loading and from inspiring English sources

The Ni2+ column was first balanced with binding buffer before sample loading and then sequentially washed with binding buffer containing 0 mM, 30 mM, 40 mM, and 70 mM imidazole.

Before sample loading, the cartridge was conditioned with methanol (1 mL) and water (1 mL).

Each assay well was first rinsed with 100 μL wash buffer (1× L-WB) before sample loading.

The membrane proteins samples were mixed with SDS sample loading buffer and incubated at 37°C for 10 minutes before loading.

Before the sample loading, the resin was washed twice with ten column volumes of binding buffer (pH 8.0, 50 mM sodium phosphate, 300 mM NaCl, 10 mM imidazole).

Airway clearance ("expectoration" of mucus simulant from the "airways"): Measurements of the weight of the MS sample loaded before and after each cough maneuver were carried out in a Mettler AE 163 microbalance.

The cells were lysed in ice-cold RIPA buffer and boiled in sample loading buffer before western blotting analysis.

Immune complexes were isolated on protein-A/G beads, washed four times with RIPA buffer and resuspended in sample loading buffer, before western blotting for AR (N20, Santa Cruz).

60 μl of column fraction or an appropriate dilution (including sample before column loading and flow through obtained during loading) was taken in a 96-well round-bottom microtitre plate (Wells 1 12).

DYNAbeads was then washed three times with 500 μL immunoprecipitation buffer before being boiled in 25 μL SDS sample loading buffer (2×).

For effective extraction, an offshoot with NH4Ac solution of high-flow rate was employed to dilute the loaded sample by a mixing tee before sample was loaded onto the C30 extraction column.