Sentence examples for before replacing the medium from inspiring English sources

The cells were incubated for 24 h before replacing the medium with medium containing nanoparticles or 20 ng/ml free Nile Red.

For mDCs with mix of adherent and suspension cells, the plate was centrifuged at 1,000 rpm for 10 min before replacing the medium to avoid loss of unattached 89Zr-labeled cells.

15 000 cells were seeded in 8-well microscopy slides and incubated for 24 h before replacing the medium with CellLight Early Endosomes-GFP at a concentration of 40 virus particles per cell.

To synchronize the cell cycle for genome-wide expression analysis, the cells were serum-starved during 7 h before replacing the medium with 20% SVF medium for 48 h.

The mixture was added to cells and incubated for 6 h before replacing the medium.

The mixture was added to the cells and incubated for 6 h before replacing the medium.

The transfected cells were seeded on Transwell chambers (Corning Life Sciences, Corning, NY, USA) before replacing the transfection medium with complete medium with or without Dex.

Cells were allowed to attach during four to five days before replacing the growth medium being DMEM supplemented with 1% antibiotics/antimycotics, 1% SP, and 10% FBS.

When orange pigment was produced after mycelia were transferred to fresh basal medium, the ammonium level showed no alteration before and after replacing the medium.

Then, the mycelium was transferred into fresh basal medium after washing with 50 mM citric acid/Na3 citrate buffer (pH 5.0), and was cultivated until the total culture time reached 96 h, including both before and after replacing the medium.

"Culture condition" describes the medium in which P. purpurogenum IAM15392 was grown before (left) and after replacing the medium (right): Basal represents basal medium, 1 mM Gln represents basal medium containing a final concentration of 1 mM glutamine, and 10 mM Gln represents basal medium containing a final concentration of 10 mM glutamine.