Sentence examples for before reconstitution from inspiring English sources

Wells were coated with ECL Cell Attachment Matrix (Upstate, Temecula, CA, USA) and allowed to dry before reconstitution in serum-free media (SFM).

They were warmed to room temperature before reconstitution, which required ~15 min.

Full activity was restored by addition of immunoaffinity purified SR before reconstitution of the detergent extract.

By contrast, allogeneic human skin grafted to 10 to 12 mice before reconstitution with patients' PBL developed pemphigus vulgaris-like lesions containing human IgG deposits.

It must be maintained at a temperature not exceeding -15 °C during shipping and storage, although it can be stored and transported at refrigerator temperature for up to 72 continuous hours before reconstitution.

Recipient mice were lethally irradiated with 2 × 600 rads using a Cs source before reconstitution.

Death in 25% of mice was secondary to infection which developed before marrow reconstitution occurred.

Syntaxin 1A and SNAP-25 (or Sso1p for yeast SNARE mediated lipid mixing), in a molar ratio of 1 1.5, were premixed, and the mixture was left at room temperature for 1 h to form the complex before the reconstitution.

Before chemical reconstitution of the Fe/S clusters in an anaerobic chamber (Coy Laboratory Products), 60 μM apo-HCF101 was reduced with 10 mM DTT in 25 mM buffer A for 1 h.

For example, while several nonionic detergents with sugar-based headgroups can catalyze IMP insertion without requiring vesicle dissolution, Triton and cholate depend on partial or complete solubilization, respectively, before efficient reconstitution can be initiated.

Surviving C. sakazakii is capable of rapidly multiplying to dangerous levels during reconstitution before feeding, and it can cause serious clinical symptoms, include necrotizing enterocolitis (NEC), bacteremia and meningitis, resulting in fatality rates ranging from 40 to 80%% (Bowen and Braden 2006; Friedemann 2009).