Sentence examples for before quantitation from inspiring English sources
This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
Before quantitation of the expression of each protein, the peak intensity of the iTRAQ signature ion was normalized, as "Method 1" of our previous study [ 72].
Before quantitation a second filtering step was performed to retain only those proteins with enough abundance (minimum abundance of 0.001) for a low-biased quantitation [ 90, 91].
Female mice were treated orally twice daily with various doses of prednisolone alone, dipyridamole, or the combination of varying doses of prednisolone with dipyridamole, for 8 weeks before quantitation of these surrogate markers of osteoporosis.
Subsequently, Sca1 antibody (1 200) Sca1/Ly6A/E (PE/Cy5) (Abcam80, Abcam) was added to the cell suspension and maintained in the dark for 20 minutes on ice before quantitation of the specific Sca1 fluorescence.
The supernatant was precipitated with two volumes of ethanol, the pellet was washed with 76% ethanol containing 0.3 M sodium acetate, dried briefly in a Speed Vac, and resuspended in 50 μl 10 mM Tris-HCl (pH 8.0) 2.5 mM EDTA before quantitation by spectrophotometry.
In samples obtained before 1996, quantitation followed storage at −80°C.
The supernatant was removed by aspiration and cells were lysed in 100 µl Promega reporter lysis buffer (E397A) and frozen at −80°C before luciferase quantitation.
Before the quantitation of proteinase 3 and cathepsin C, GCF samples were eluted from the strips by placing them in 2 mL phosphate buffered saline by shaking the tubes on an ELISA plate shaker for 45 minutes.
This method employed ultrasound-assisted emulsification microextraction (USAEME) before identification and quantitation of the parabens via on-line acetylation gas chromatography-mass spectrometry (GC MS).
Briefly, 228 copies of an internal standard plasmid were added to all DNA extracts to provide a reference for quantitation before PCR amplification of a 93 base-pair segment of exon 7 of TP53 encompassing codon 249.
