Samples with pyrite suspensions were filtered (Millipore 13 mm 0.45 μm PVDF) before quantification of remaining adenine by UV absorption at 260 nm.
Following homogenization of the sample, dilution and plating, the plates were incubated for 48 h at 21 °C before quantification of CFU/g meat.
Biofilms were incubated in candle jar for 6 h at 37°C before quantification.
Beads were extensively washed with binding buffer before quantification of bounded peptide by Cerenkov counting.
Cells were left for 2 days following transfection, before quantification of AKT1 phosphorylation or PS staining.
High purity hydrochloric acid was used for mercury dilutions before quantification by inductively coupled plasma optical emission spectrometer (ICP-OES).
2) Library release from beads before quantification used the heat-release protocol advocated by Maricic and Pääbo [28].
The BCA Protein Assay (Pierce, Rockford, IL) was performed to measure total cellular protein from each of the cell groups under consideration just before quantification of cytokine release.
After PCR amplification, emulsions were broken using butanol, and the beads were washed, enriched, and terminal transferased before quantification and deposition onto a slide for sequencing.
After PCR amplification, emulsions were broken using butanol, the beads were washed, enriched, and terminal transferased before quantification and deposition onto a slide for sequencing according to manufacturer's instructions.
The PCR products were desalted by ethanol precipitation before quantification.
