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Clones proliferated for one month before proliferation spontaneously slowed and morphological changes occurred in most cells.
Cells were treated (or not) with gemcitabine for 24 h before proliferation was assessed by luciferase activity.
Cytotoxicity must be of a certain magnitude and be sustained for a certain period of time before proliferation is stimulated.
In other words, the metastasis-suppressed cells complete every step of the metastatic cascade before proliferation at the secondary site to form macroscopic metastases.
However, the differences in gene expression above and below the median of survival correlate well with different gene signatures identified before (proliferation signature) as well as with the new ones described in our study (non-proliferative signatures, see below).
We model the behavior of a system comprising two CD4+ T cells that have been activated by their cognate antigens, but before proliferation takes place (within ~30 hours after activation).
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That's what we did back in the day, before the proliferation of selfie sticks, before hotels started handing them out for free like umbrellas and vendors at the Colosseum and the Eiffel tower added them to their roster, in addition to fractal versions of the monuments themselves and $4 water.
One could argue, in reference to Antiquity, that such time travel was only reserved to the Gods.... Never before was the proliferation of portraits so abundant but also so crucial in the forming of opinion of us by others.
This started before the proliferation of bands and self-published zines that carried out the collective's ideas.
At a time before the proliferation of best-seller lists or well-stocked bookstores, the club grew explosively, signing up over 100,000 members in its first two years.
An audience of a certain age agrees to gorge retrospectively on the illusions of youth from an era long before the proliferation of viral gossip and reality television.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com