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RNAse A (100 µg/ml) and propidium iodide (10 µg/ml) were added to the cells before processing on a FACScalibur system and CellQuest software.
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One of the weaknesses of our study was that an extended extraction protocol was required before processing on an automated platform, increasing manual complexity and turn-around time.
After washing with phosphate-buffered saline, cells were stained with propidium iodide (1 mg/mL) and RNaseA (250 μg/mL) for 30 minutes before processing on BD FACS-Calibur (BD Bioscience).
Quality control was performed for all samples before processing them on the Illumina arrays.
Specimens were then collected throughout the week and refrigerated (before processing) until delivery to the laboratory on a weekly basis by secure motorized transport.
Before processing, samples were cut on dry ice to give approximate weights between 15 and 25 mg.
Meng says processing grain on a smaller scale requires infrastructure that existed before the Industrial Revolution but no longer does.
TGA curves of PLLA, PPA and a-PHB before processing.
For analysis of potential circulating metabolites, blood samples from the baseline group were collected into heparinized tubes and immediately stored on ice before processing.
(a) Before processing.
Samples were immediately transferred to a sodium heparin 10 ml blood collection tube (BD Vacutainer, Franklin Lakes, NJ, USA) and stored on ice before processing.
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