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Membranes were blocked with 5% natural nonfat dry milk before probed with anti-PCNA antibody (FL-261 sc7970, rabbit polyclonal 1∶400, Santa Cruz Biotechnology, CA, USA).
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Plaque samples in the two most posterior teeth (mesiolingual in the upper jaw and mesiobuccal in the lower jaw) were collected before probing.
Restore PLUS Stripping beffer (Thermo Fischer #46430) was used to strip blots of in between antibody sets, before probing with new ones.
Before probing an employee's thoughts, particularly those involving off-the-job ideas, an employer must be certain the research is germane to actual work.
Indeed, if Merson wanted to criticise anybody's tactics it ought to be those of the Anderlecht manager, Besnik Hasi, who left it until his team were 3-0 down before probing with any real purpose some very obvious Arsenal weaknesses.
"We photoexcited the iron atoms in the protein's active site which contains an iron-rich compound known as heme with an ultrafast laser before probing it with the LCLS X-ray pulses at different time delays," says Alonso-Mori.
No signals were obtained with an array where proteins were heat-denatured before probing with RNA, indicating that RNA interactions must derive from active proteins.
To confirm that the binding of HA-Fc observed in these studies was SA dependent, tracheal airway sections were exposed to recombinant neuraminidase before probing with HA-Fc.
Tool swapping was examined by identifying all trials on which a subject extracted a tool but discarded it for another one before probing for food.
All three subjects nearly always probed for food before probing for tools in Primary trials, when the food was within reach of the tabletop tool (Corbeau: 89%; Pierre: 92%; Uék: 73%).
The sections were treated with 80% formic acid for antigen retrieval before probing with monoclonal antibody 1E8 that recognizes an epitope between residues 18 and 22 of Aβ (Glaxo SmithKline, UK).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com