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Since linear polyphosphates must undergo hydrolysis (either biotic or abiotic) to ortho-P before precipitating as a mineral phase with either Ca or Fe (pH dependent), they can act as a slow release fertilizer [7].
The suspension was stirred at 4°C for 1 hour before precipitating in 10 L of water.
The suspension was stirred at 4°C for 1 h before precipitating in 10 15 L of water.
Western blot (Invitrogen) was used to verify protein expression and quality before precipitating the media with 80% saturated ammonium sulfate (Fisher).
Western blot (Life Technologies) was used to verify protein expression and purity before precipitating protein from the media with 80%% saturated ammonium sulfate (Fisher).
Briefly, assay mixtures were incubated for 45 minutes at 70°C in thin-wall PCR plates before precipitating the radiolabeled transcripts by the addition of ice-cold TCA solution.
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As in her previous novel, "A Day at the Beach," in which the events of 9/11 set in motion another wealthy Manhattan family's crisis, the book's tragedy seems to have been in progress long before the precipitating events occur.
Then 1 μg DNA was treated with a sodium bisulphite solution at 50°C for 4 hours before ethanol precipitating and eluting in 50 μl tris-EDTA buffer.
It has been proposed that TPP rapidly hydrolyzes in the soil solution before adsorbing or precipitating with soil surfaces, but in model systems, TPP also adsorbs rapidly onto mineral surfaces.
As before, it was precipitated by a huge international economic crisis.
The aqueous layer was removed and lyophilized before precipitate removal by resuspension in 600 μL of sodium phosphate buffer in D2O (0.1 M, pH 7.4), containing 60 μL of 0.1% sodium TSP, and centrifugation at 14,000 rpm at 4°C for 8 min. The resulting solutions were transferred to 5-mm NMR tubes, and NMR spectrum was acquired with water signals suppressed by presaturation, as described above.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com