Sentence examples for before plunge from inspiring English sources

Briefly, GTPγS microtubules were grown from quantum dot-labeled GMPCPP seeds in the presence of 2 mM GTPγS, 12 μM tubulin, and 45 μM Mal3143 at 37°C for 5 min before plunge freezing (see Figure S1A).

Extended Experimental Procedures Cryo-EM Imaging for Protofilament Number Analysis GTPγS microtubules were grown from quantum dot-labeled GMPCPP seeds in the presence of 1 mM GTPγS and 20 μM tubulin at 37°C for 30 min before plunge freezing.

For Mal3143-GDP micomplexes comicrotubulesrotubules were grown from quantum dot-labeled GMPCPP seeds in the presence of 2 mM GTP, 10 μM tubulin, and 55 μM Mal3143 at 37°C for 1 min before plunge freezing.

GTPγS microtubules were grown from quantum dot-labeled GMPCPP seeds in the presence of 1 mM GTPγS and 20 μM tubulin at 37°C for 30 min before plunge freezing.

Adsorb for 30 sec Float the grid in the 16% ammonium molybdate droplet (sample side facing droplet) for 60 sec Blot with filter paper, air dry 1-3 sec, and plunge into liquid ethane Both the length of blotting and the amount of time the sample is allowed to dry before plunge freezing is critical for obtaining the optimal thickness of vitrified ice.

Throughout the continent, it inhabits diverse aquatic habitats where it typically perches over clear open water before plunge-diving for prey¿chiefly fish, but also other aquatic animals such as crayfish.

Wisely, he got his feet wet before plunging in.

Before plunging into the studio business, Tiemann made his mark in the high-tech field.

Wouldn't it be great to try your career on for size before plunging in?

They gather up the space with curving arms before plunging into it.

During this period the economy initially expanded, before plunging into recession during the financial crisis.