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For experiments involving conditioned macrophage media and tumour necrosis factor (TNF -α applicaTNF -αdissociapplicationlls were spun through an 11% bovine serum albumin cushion before plating, to redissociatedesence of non-neuronal cells.
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We used a ~1-μm diameter fiber structured GdAlO3 Ce (GAP) /α-Al2O3 scintillator plate to reduce the light spread.
The same incubation times and temperatures for both antagonist and fluorescent ligand incubations were used; however, all ligands were washed out before imaging on the confocal plate reader to reduce fluorescence background levels.
As always, please plan to bring reusable plates and cutlery to reduce waste.
The initial interpolation of the grids was scaled to a relatively coarse resolution (∼111 km2) before a thin-plate smoothing spline algorithm was applied to reduce the surfaces to various finer resolutions that were validated against historical weather station data multiple times to reduce error associated with interpolation [76].
Biofilm swab samples were placed on a lawn of Escherichia coli for detection of ameba and treated with diluted acid (0.1 mol/L KCl, 0.005 mol/L HCl) to reduce the number of non- Legionella bacteria before plating.
Treatment of MCF-7 cells for 2 hours with 1 μM doxorubicin in monolayer culture, before plating them into soft agar, reduced the CFE by 61% (P < 0.05 by unpaired Student t test; Figure 4a).
To reduce the effect by the position of plates in the growth chamber, plates were moved every two days.
To reduce intraexperimental variability, data were normalized on a plate-by-plate basis.
To reduce waste, paper cups and plates are not allowed in the cafeteria, except at the Starbucks stand.
"I never liked restrictor-plate racing," Stewart said, referring to the devices used to reduce speeds.
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