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aTc was added to liquid M9-glycerin medium at a concentration of 500 ng/mL to stringently repress gene expression before plating.
Before plating the cells, cell culture dishes or plates were coated with rLN511E8 (0.5 μg/cm2).
Chemical pre-treatment before plating increases the substrate surface roughness significantly.
As shown in this work the adhesion can be improved by a more thorough etching of the oxide before plating.
All cultures were prepared in a selective broth medium for 18 to 24 hours before plating onto sheep blood agar.
Then, in a nod to the scientific basis of modernist cuisine, he used a pipette to decorate a plate with beet puree before plating the single raviolo.
RNA was isolated before plating cells (growth), at early and completed aggregation, tipped mound and slug stages, and at early and completed fruiting body formation.
All the samples were vibrated to make cells fully dispersed before plating.
The preformed pellets/injectable hydroxyapatite-coated coverslips were autoclaved before plating placental-derived MSC.
Arsenic contaminated soil samples were enriched and diluted before plating on both autotrophic and heterotrophic media supplemented with As (III).
For spore counts, the serial dilutions of culture samples were pasteurized at 80°C for 15 min before plating.
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