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The contigs were checked and edited manually if necessary before phylogenetic analysis and deposition in GenBank.
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For ligand-receptor inference, the first 20 amino acids (leading peptide) of all chemokines were removed before the phylogenetic analysis and chicken CCLs were divided into two groups, one group located on Chromosomes 4 and 19 and the other from other chromosomes.
Additional corrections were made using McClade 4, v. 4.01 [33], including end trimming to remove areas of missing data, and further alignment of indels (insertions and deletions) before phylogenetic analysis.
Alignments were checked manually and ambiguously aligned regions were removed before phylogenetic analysis.
Regions where homology was doubtful were manually removed from the data sets before phylogenetic analysis using the NET program of the MUST package (Philippe 1993), and phylogenetic trees were computed with PHYML (Guindon and Gascuel 2003) and MrBayes (Ronquist and Huelsenbeck 2003; Huelsenbeck and Ronquist 2001).
Sequences were manually aligned in Sequencher 4.5 against a subset of the existing Acropora Pax-C intron and mitochondrial control region sequences [2], [5], [9] before phylogenetic analysis in a Bayesian statistical framework in Mr Bayes 3.1.2 [10].
Multiple alignment encompassing database and PCR sequences were trimmed at the same length before phylogenetic analysis.
Ambiguous regions were deleted from the alignment before phylogenetic analysis, for a final length of 678 bp.
Sequence alignments were performed (manually and/or with ClustalW) before phylogenetic analysis and/or Weblogo3 analysis [ 52].
Columns with gaps were eliminated before phylogenetic analysis.
Sites with ambiguous alignments were removed before phylogenetic analysis.
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