Sentence examples for before performing the pull-down from inspiring English sources

Firstly, immunoblotting on whole cell lysates collected before performing the pull-down assay showed similar Ras-expression levels in EqS02a, EqS02aE5 and EqS04b.

For the SFB-tagged protein, streptavidin beads were used to perform the pull-down assay followed by Western blotting analysis.

EMV performed the GST pull-down assays.

GW performed the compound pull-down and ubiquitination assays.

The amount of GFP-RAB-3 input in each experiment was equalized before the pull-down assay.

A strong interaction was detected between Robo1 and Robo2 when the pull-down was performed in either direction.

The pull-down assay was performed using MagneGST Pull-Down System (Promega# V8870).

The pull-down assay was performed following a previous procedure.

In-vitro translation of Foxo1 and the pull-down assay were performed as described previously (Cao et al, 2006).

The pull-down experiments as per Figure  6B and C were performed as previously described (66).

Co-immunoprecipitation (Co-IP) experiments were performed in S2 cells to pull down the endogenous Yki or Sd protein using antibodies, and the pull-down products were sent for MS analysis.