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The duration of exposure to chlorine dioxide was either 60 min or 24 h before neutralization.
The hydrolysate was devoid of solids through paper filtration before neutralization with calcium hydroxide and sodium hydroxide.
However, sulfate remains constant and can be used to approximate the degree of acidity present or that was present before neutralization took place.
Before neutralization, the AChE586-599 spectrum did not exhibit any strong positive bands, indicating the absence of conformational restriction of the aromatic side-chains (Figure 2C, left panel).
SAX-HPLC sample preparation usually consists of incubating the reaction mix or cells with percloric acid (1 2 M) for 20 30 minutes on ice before neutralization [20], [25].
Washed WT and PF4−/− mouse platelets were isolated and resuspended in Tyrode's buffer as we have described [55] and activated with 0.5 U/mL of thrombin (Sigma) for 10 mins before neutralization with hirudin (Sigma) and centrifugation.
Although the two pseudovirus strains tested in this study represent tier 1 viruses and much more work is certainly needed before neutralization of tier 2 viruses may be achieved, the reported data indicate that an anti-HIV-1 epitope-based vaccine approach is possible.
All samples were acid-treated before neutralization in the presence of biotinylated and sulfo-tagged ALX-0061.
Total soluble sugars were determined by the same method boiling an aliquot of PCA extract for 1 h before neutralization.
After a vortex mix, the suspension was incubated for 15 min at room temperature before neutralization with 15 ml of phosphate buffer.
Hydrolysis was stopped by the addition of iodoacetate sodium salt (10 mM final concentration) before neutralization with Tris-HCl buffer pH 8.0.
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