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Material was left in staining solution for at least one week, but usually up to a month before mounting on slides.
The sample powders were deposited on a carbon tape before mounting on a sample holder and gold coated for EDAX.
To operate the tool, a substrate (of up to 150 mm square) must be pre-coated in UV-sensitive resist before mounting on the vacuum port of the hotplate base.
Staining was performed on free-floating sections before mounting on superfrost slides (Fisher Scientific, USA).
Before mounting on glass slides and being treated with 5% gelatine, the sections were kept in demineralised water (pH 8) for a minimum of 20 minutes.
Following immunostainings, slices and sections, still including culturing membranes, were washed with KPBS + Triton X100 and KPBS before mounting on microscopy slides using DABCO fixation.
Similar(29)
Sealants were formulated, batched and exposed to various aging conditions before mounted on ATR spectroscopy apparatus for monitoring.
One hour following tMCAO, animals were anaesthetized as mentioned before, mounted on the animal holder of the scanner and the body temperature was kept maintained.
In all cases, tissue sections were washed again as before, mounted on glass slides, dehydrated, treated with Vectashield (Vector Labs, Burlingame, CA) and sealed with a coverslip and nail polish.
The cells were washed 4 times with PBS-T, 7 min each time before mounted on slides with VectoShield with DAPI Vector Laboratoriess, Burlingame, CA).
Paraffin blocks of MI samples (fixed overnight in phosphate-buffered formalin (4%, pH 7.2)) were stored in RNase-free sealed boxes at 4°C until cutting (5 μm), before mounted on Super Frost Plus slides (Bergman, Lillestrom, Norway) and dried overnight at 30°C.
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