To ascertain that species misassignment and potential biases in read loss does not cause S expression values to lose accuracy, we mixed pairs of human and mouse samples in silico and compared expression values before and after mixing the samples and using S (Fig. 1e, f), with good correlation.
Secondary outcomes were measured by (1) changes in urine appearance (before mixing the urine sample with the staining solution, the visual appearance of each urine sample was noted as being clear, cloudy or blood stained) before and after treatment, (2) changes in haematuria (using Hemastix®) before and after treatment and (3) patients' opinion towards the different treatment arms.
Reactions were initiated by mixing the samples before incubation at room temperature.
The concentration of each labeled peptide was measured before mixing the two different fluorophore-labeled samples using cTCCD as previously described.
Have the rose petal ready before mixing the cocktail.
Additionally, some MID-barcodes produced more reads than others even though an attempt was made to mix the samples in equimolar amounts before sequencing.
A total of 300 μL of distilled water and 20 μL of 30% zinc sulphate (ZnSO4) (Sigma-Aldrich, St .Louis, Mo, USA) were added before mixing and centrifuging the samples for 5 min.
Before mixing with the nitrocellulose-based ink, the stored Cu NWs sample needed to be purified from PVP and DEHA.
Although in this experiment, starting with less than 1 μg of total RNA did not produce enough biotinylated cRNA for hybridization under the normal protocol, a minor change (using vacuum evaporation to concentrate samples before cRNA synthesis, and mixing the minimum 200 μl hybridization volume) should allow use of 1 μg samples with the standard protocol.
At the laboratory, the sample was vigorously mixed and allowed to settle with a magnet pressed against the outside of the container positioned above the height of the sediment surface before mixing.
