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However, the bones were decalcified by formic acid before microCT analysis of the vascular bed [39].
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MicroCT analysis of 8-week-old Arf-/ and Arf+/+ littermates demonstrated significant increases in bone mineral density, trabecular bone volume and trabecular number and thickness in Arf-/ mice (Figure 1a).
MicroCT analysis of Lrp4 deficient mutant mice revealed shortened total femur length, reduced cortical femoral perimeter, and reduced total femur bone mineral content (BMC) and bone mineral density (BMD).
The same trend was found in a microCT analysis of the L5 vertebra.
MicroCT analysis of the alveolar bone of the mandible in Casp7−/− mice showed a statistically significant (P=0.024) decrease in bone mineral content (BMC).
To determine feasibility of histologic evaluation following microCT analysis, sections of aortas from Ldlr-1KO-AD mice preselected by microCT and en face Sudanophilia were assessed.
For microCT analysis, right femurs of both Dex- and saline-treated mice were fixed with 4% paraformaldehyde and analyzed by Scanco Medical CT-40 instruments.
Additionally, microCT analysis confirmed uniform distribution of HA throughout the mineralized scaffolds, whereas no X-ray absorption was detected for non-mineralized control scaffolds (Fig. 1B).
Thus, data from immunohistochemistry and microCT analysis pointed to the role of caspase-7 in the formation of the alveolar bone.
In this study, we use resin casting and X-ray microtomography (microCT) analysis to address the role of Notch signaling in the maintenance of formed IHBDs upon chronic loss or gain of Notch function.
Selected suture samples underwent MicroCT analysis to determine the degree of fusion, as previously described [ 73].
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