Sentence examples for before loading the sample from inspiring English sources

Before loading the sample onto the SDS-PAGE gel, samples were boiled at 100 °C for 5 min.

Before loading the sample to the column of microscope, some preparation was needed for the instrument and for loading the sample.

The collected HPLC fraction can only be diluted with water into a 20 mL syringe before loading the sample on a C18 cartridge.

The cartridge was preconditioned with 3 6 ml of methanol and then with 3 6 ml of ultrapure water before loading the sample.

A 0.45-μm syringe filter was used to filter dust from the samples before loading the sample to the Zeta PALS.

The optimal cleaning process for EBL masks before loading the sample in the PA-MBE chamber consisted of 30 min immersion in pyrrolidone at 80°C followed by a hot isopropanol rinse and abundant hot water rinse.

Before loading the samples into the UHV evaporation chamber, their surface was refreshed in diluted HF.

Double-layer filter papers (110 mm, Filtech®, Australia; pore size 2.3 μm, 0.06% ash) were placed on the bottom of the funnel (Buchner 110 mm, Kartell®, Italy) before loading the samples.

After 1  μl of each PCR product was diluted with 7  μl of DNase-free, RNase-free distilled water, 0.1  μl of each diluted sample was added to 0.7  μl of formamide and 0.3  μl of ROX 400HD size standard and denatured at 95°C for 5 min before loading the samples into the Applied Biosystems 373 DNA sequencer (Applied Biosystems).

The chips were initially filled with ethanol then rinsed with buffer before loading the different DNA ladders and the samples.

The SDS-Protein complexes formed when the samples were boiled before loading the gel migrated to their expected positions in the gel based on their molecular weights (Figure 5A).