Sentence examples for before loading onto the from inspiring English sources

The proteins were isolated at 2 or 6 h after exposure and labeled with Cy3 for the irradiated cells and with Cy5 for the control samples before loading onto the protein microarray chips.

Protein samples must be free of other charged molecules before loading onto the gels.

Samples at same concentration in terms of OD = 5 were treated with buffer and heated at 100 °C for 5 min before loading onto the gel.

For this reason, people tend to drink in excess before loading onto the coach buses -- I need not say more.

The solution was then filtered using a 0.45-µm syringe filter (Millipore, Cambridge, ON, Canada) before loading onto the column.

Samples were mixed 1∶1 (v/v) with sample buffer supplemented with 5% mercaptoethanol, and boiled for five minutes before loading onto the 10% polyacrylamide gel.

Samples are incubated at 30 °C for 10 min before load onto the gel. 1) Freshly prepare 10 mL gel solution per gel and mix on the roller mixer for 2 h at room temperature.

Sequencing primer and polymerase were added to the final enriched spheres before loading onto an Ion 316 chip according to the Ion PGM™ 200 sequencing kit protocol.

Sequencing primer and polymerase were added to the final enriched spheres before loading onto an Ion 318 chip according to the Ion PGMTM 200 sequencing kit protocol.

For subunit purification, 80S ribosomes were exchanged into dissociation buffer (20 mM MES-KOH [pH 6], 600 mM KCl, 8 mM Mg+2 -acetate, 1 mg/ml heparin, 0.1 mM PMSF, 0.1 mM benzamidine, and 2 mM DTT) before loading onto a sucrose gradient in the same buffer and centrifuged for 19 hr at 28,500 rpm in the Ti25 rotor.

The eluted fractions containing WRN were loaded onto a HiTrap Desalt 5 ml column to exchange the buffer before loading onto a 1 ml Resource Q column for removal of remaining contaminants as described previously [60].