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All libraries were normalized to 10 nM before loading on the Illumina sequencers.
The aim of this study was to evaluate the influence of different healing times before loading on the stability of implanted titanium mini-implant.
To resolve GFP-like proteins in the native state, the samples were not boiled before loading on the gel, and visualized after the run by their native fluorescence.
Excess dye was removed using Ambion's AminoAllyl MessageAmp II aRNA Kit (Ambion Cat# AM1753) by pooling the Cy5 and Cy3 labeled samples before loading on the clean-up columns.
The identity of polyP was confirmed by its complete degradation when TRPM8 was incubated with 2 µg/ml scPPX1 (Wurst et al., 1995) for 3 h at 37°C before loading on the gel (Fig. 4A, lane 3).
On the other hand, when the samples are not heated before loading on the SDS-PAGE gel, there is a low but significant labeling with the 32P radiolabel of the KaiCTae mutant protein that is slightly above the level in KaiCTaa (Fig. 4B, right panel).
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Some collapsed immediately while others still had some blastocoelic fluid at the end of the incubation period before loading on to the carriers.
The aqueous mixed gellan samples were heated to 90 °C, before loading on to the pre-heated plate of the rheometer.
Membranes were loaded with genomic DNA extracted from 2 individuals, one the single standard loaded on each membrane, the second a test plant; before loading on blots the DNA was fragmented by passage through a narrow gage needle.
If enough quantity of input is provided or enough samples are pooled, EXPRSS samples do not require any amplification before loading on to the Illumina flow cell, as they have adapters required to bind in the flow cell.
Whole-cell lysates were subjected to TCA precipitation before loading on to the SDS gels.
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