Before each experiment, larvae were collected in clean water, in the presence of T. latifolia and put at room temperature under red light for 1 h.
Samples of larvae were collected before metamorphosis (up to day 12 postfertilization; 6 7 mm) and during and after metamorphosis (from day 14 up to day 23 postfertilization; 8 9 mm).
Late third-instar larvae were collected 6 12 hr before pupariation.
Middle third-instar larvae were collected 24 36 hours before pupariation.
Larvae were collected from the food medium immediately before the experiment and gently rinsed in tap water for cleaning.
Meanwhile, WBC larvae were collected in Lowville (Lewis County) this week.
A total of 177 larvae were collected from the wild in England, France, Germany, Slovakia and the Czech Republic, and then tested for the DNA of four insects.
Percutaneously migrated larvae were collected using the PERL chamber system and ovine skin in vitro.
A total of 267,013 larvae were collected in 31.1% of the inspected containers.
Using a moistened paint brush, third-instar larvae were collected and allowed to habituate until observation of progressive movement (~45 s).
