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Male and female mice were randomly allotted and treated with a single dose 30 min before intraperitoneal injection of phenol red solution (5%% in saline solution, w/v, 0.1 mL/10 g body weight).
At 17 weeks of age, mice were fasted for 16 h before intraperitoneal injection of a 10% D-glucose solution (1.0 g/kg).
Mice were given CJ for 1 week before intraperitoneal injection of LPS.
Rats were orally treated with MAK (1 g/kg) or fluvoxamine (10 mg/kg) 60 min before intraperitoneal injection of 5-HTP (200 mg/kg).
Different extraction fractions from TS leaf extracts (TSL1 to TSL5) were administered orally 1 h before intraperitoneal injection of acetic acid.
To this end, CWE was orally administrated to mice for 6 days before intraperitoneal injection of LPS and the release of serum TNF-α and IL-6 was measured by ELISA.
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BALB/c mice were anesthetized with 4% isofluorane before the intraperitoneal injection of 125 mg/kg body weight of luciferin (sodium salt; Promega).
Four-week infusion of 1400W [an inhibitor of inducible nitric oxide synthase (iNOS); 50 μg/kg/hr] and diphenyleneiodonium (DPI; a NADPH oxidase inhibitor; 5 μg/kg/hr) or vehicle (phosphate-buffered saline containing 6% DMSO) via osmotic minipumps (0.25 μL/hr; 90 μL total capacity; replaced once) was started 30 min before the intraperitoneal injection of NS/LPS.
For the assessment of writhing behavior, the test materials, the reference analgesic drug aspirin (200 mg/kg) or vehicle control (1% SMC) were orally administered to all mice 30 min before the intraperitoneal injection of 0.6% acetic acid (10 mg/kg body weight).
For drug administration, acetaminophen was dissolved in normal saline, whereas dabrafenib and vemurafenib were dissolved in pH 8.0 distilled water containing 0.5% HPMC and 0.2% Tween 80. Overnight-fasted mice were given dabrafenib (300 mg/kg or 100 mg/kg) or vemurafenib (300 mg/kg) by oral gavage 1 h before the intraperitoneal injection of 300 mg/kg acetaminophen.
CDDP was provided by the Pharmacological Department of our institution; it was diluted in sterile serum before intraperitoneal injection.
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