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We continue to argue that the much more likely process is one in which, as in our hypothesis, the internalized cell was, at the beginning, surrounded by an ionic milieu approximately the same as that it enjoyed before internalization, and thus, from the internalized cell's point of view, it was still outside.
It might be advantageous to choose a low-affinity minigastrin for multimerization because this increases the possibility that more than one binding event per molecule can occur before internalization prevents further multivalent interactions.
HIV-1 particles and physiological ligands that bind the RGG domain of nucleolin (Midkine, pleiotrophin, lactoferrin) induce aggregation of surface nucleolin, before internalization into various cell types by an active process via lipid raft microdomains [7], [10], [17], [18], [19].
We hypothesize that cell wall loss occurred before internalization.
Third, Cbl association with and ubiquitination of EGFR occur at the cell membrane before internalization [ 22].
If this were the case, infection in the presence of BafA might be rescued if EV membranes were artificially disrupted before internalization.
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In the first stage of endocytosis, NDs in the medium will anchor to plasma membrane before their internalization by cells.
Mannosylated SLNas exhibited physical properties suitable for alveolar macrophage passive targeting, adequate rifampicin payloads (10 15%), and feasible drug maintenance within SLNas along the respiratory tract before macrophage internalization.
Pyroptosis following FlaTox-induced activation of the NLRC4 inflammasome was also associated with cell swelling before cellular internalization of Sytox Green was observed (Fig. 6c, d, Supplemental Fig. 7b and Supplemental Movie 14).
Since temperature has a differential effect on BODIPY-lipid micrometric assemblies, 20°C was selected as a compromise: (i) for which all three types of domains could be readily observed on CHO cells; (ii) that could be rapidly reached by warming up; and (iii) at which imaging could be rapidly performed before significant internalization would take place.
Therefore, termination of TCR-mediated signaling occurs before TCR internalization.
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