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The first frame was acquired before injection of a paramagnetic contrast agent (gadopentetate dimeglumine, 0.1 mmol/kg body weight, Magnevist™, Schering, Berlin, Germany), which was immediately followed by five other measurements.
In mice implanted s.c. with Lewis lung carcinoma, priming with 5 mg kg-1 methyl-CCNU 2 days before injection of a very high (35 mg kg-1) dose significantly increased the lifespan as compared to treatment with the high dose alone or with high-low dose combination.
T1-T4 were inserted on post infection day (PID) -4, and T5+T6 were inserted PID 0. Before injection of a transponder, the skin area to be involved (10 × 10 cm) was shaved and surgically cleaned with 70% ethyl alcohol and 5% iodine, respectively.
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Before injection of the contrast agent, an anatomical white light image was obtained.
The column was equilibrated for 5 min with solvent A before injection of the next sample.
Before injection of the cells, all mice received a 0.72-mg pellet containing 17β-estradiol in a 60-day release preparation (Innovative Research of America, Sarasota, FL, USA).
Nicotinamide was injected intraperitoneally 15 min before injection of Streptozotocin intravenously.
The mice were given dexamethasone or escin before injection of LPS.
All stimuli were started 30 s before injection of the radiotracer.
There are no significant differences in power spectra between control animals before injection of DMSO, after injection of DMSO, and experimental animals before injection of capsazepine.
To account for background and nonspecific luminescence, mice were imaged before injection of luciferase.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com