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Before injection into mice, the eluate containing 68Ga-DOTA-exendin-3 was diluted with PBS containing 0.5 % BSA to a final ethanol concentration of less than 10%%.
The fractions containing 68Ga-DOTA-exendin-3 (retention time 14 15 min) were collected and diluted with PBS containing 0.5%% bovine serum albumin (BSA) to a final ethanol concentration of less than 10%% before injection into mice (injection volume 0.2 ml).
Before injection into mice, T1 cells were analyzed by flow cytometry for purity of preparation.
In cell-mixture experiments, cells expressing the GFP or the Cherry proteins were mixed before injection into mice.
The DC were incubated for 90 min with 50 µg/mL peptide and washed extensively with PBS before injection into mice.
RNA was extracted from HMECs before injection into mice and from tumours in the fat pad with the use of an RNeasy kit (Qiagen).
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To confirm this result in C57BL/6 mice, we added 30 μM NQDI-1 to the BMP/Matrigel mixture before injection into WT mice and repeated the ectopic ossification experiments.
To determine if targeting L1CAM was sufficient to reduce glioma stem cell tumor growth in vivo, we targeted L1CAM in glioma cells before injection into immunocompromised mice or directly in established tumors.
Cells were transferred to a 15 ml conical tube containing fresh medium and then washed and re-suspended in 100 µl of medium before injection into nude mice.
Before injection into recipient mice, the platelets were incubated for 10 min with the fluorochrome CFSE, Molecular Probes, Eugene, OR) at a final concentration of 90 μM.
To determine the contribution of chemokine-driven migration of neutrophils to the bone marrow, liver, and spleen, In-labeled neutrophils were treated with pertussis toxin before injection into recipient mice.
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