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After formation, the stable droplets are stored temporarily in a syringe before injection into a second chip along with a surfactant-containing aqueous carrier phase to form a double emulsion.
The extract was mixed with two spoons of Na2SO4, filtered through a 0.45-μm PTFE syringe filter directly into a 10-mL GPC vial and concentrated to approximately 2.5 mL under a gentle stream of nitrogen before injection into a GPC system (AccuPrep MPS™ GPC system, J2 Scientific, Columbia, MO, USA, equipped with a Express™ column (J2 Scientific).
Briefly, dry samples were methanolized in methanol/HCl 0.5 N, N-reacetylated, and trimethylsilylated in a mixture of N,O-Bis trimethylsilyl trifluoroacetamide aN,O-Bis trimethylsilyl trifluoroacetamideo andas chromatograpyridinea BPX70 12 m × 0.22 mm diameter column (Chrompack).
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The solutions were filtered through a 0.45μm membrane filter before injection into the HPLC system.
The supernatant was passed through a 0.45 µm filter before injection into the column (Lichrospher®, C18, OD 2.5 µm, 250×4 mm I.D).
All samples were filtered through a 0.2-μm nylon membrane before injection into the HPLC instrument.
After forced degradation process, each solution was filtered through a 0.20μm PTFE syringe filter before injection into the HPLC system.
Externally generated ions are accumulated in a linear octopole ion trap before injection into our 9.4 T Fourier transform ion cyclotron resonance (FT-ICR) mass analyzer.
Then, the filtrate were stored at 4 °C and filtered through a 0.22 μm membrane filter before injection into the LC system for analysis.
For co-electroporation, NICD and Hes1-/Hes5-siRNA vectors in a 1∶1 ratio were mixed before injection into neural tube.
All the solutions were stored at 4°C and filtered through a 0.22 μm membrane filter before injection into the UPLC system for analysis.
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