Sentence examples for before imaging the medium from inspiring English sources

Before imaging, the medium was replaced with pre-warmed Opti-MEM (GIBCO-Invitrogen).

Before imaging, the medium was changed to CO2-independent medInvitrogenrogen), and the cell culture chamber was placed onto a heated sample stage within a heated chamber (37°C).

An hour before imaging, the medium was changed to CO2-independent medium without phenol red (Invitrogen) supplemented with 10% FCS, 0.2 mM L-glutamine, PenStrep and 1 mM Na-pyruvate (all Invitrogen). 1 μg/ml cycloheximide (Sigma) was added to avoid new synthesis of SMC1-EGFP.

Before imaging, the culture medium was replaced with CO2-independent medInvitrogenrogen) containing 10% FBS.

Cells from passages 6 and 28 were seeded on glass coverslips, and for DASPMI staining they were incubated with 3 μM DASPMI for 40 min. Before imaging, the cells were rinsed with culture medium to remove extracellular DASPMI.

Before imaging, the cells were washed with 2× SSC and covered with 25 μL of Vectashield mounting medium (Vector Laboratories, Burlingame, CA) and an 18 × 18 mm no. 1 coverslip.

Styryl-9M in DMSO was diluted in serum free medium as described above and applied to both sides of the pinna 16 hours before imaging the tumor colonies.

Before imaging, the substrate was rinsed with deionized water.

Before imaging the cells were transfered to hibernate E media.

Cells were plated on 35 mm coverslip-bottom dishes (MatTek, Ashland, MA) 18∼24 hrs before imaging and the medium was changed to have 2% FBS a few hours before imaging.

One hour before imaging, the culture medium was replaced with L15 phosphate buffered medium (Invitrogen), and the cells were placed on the microscope stage to prevent focus drift due to differences in temperature between the Lab Tek chamber and the objective.