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At 45 minutes before imaging, mice were injected intravenously (i.v).v
The gel was then dried before imaging using a Typhoon variable mode imager (GE Healthcare).
All samples were blinded before imaging and quantification.
Bismuth subsalicylate was administered orally 24-72 h before imaging.
Before imaging, samples were extensively washed in PBS + 0.2% Tween-20.
Chromatin was briefly counterstained with Hoechst (20 μg mL−1) before imaging.
The membrane was then allowed to recover in fresh growth medium for 15 min before imaging.
Feather samples were carbon-coated before imaging.
All samples were carbon coated before imaging.
Anaesthetics were injected 10 min before imaging.
Before imaging, the substrate was rinsed with deionized water.
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