Sentence examples for before further purification by from inspiring English sources

The eluted solution was concentrated to about 5 mg/mL before further purification by gel filtration (Superdex-200, GE Healthcare) in buffer containing 0.02% DDM, 30 mmol/L HEPES, pH 7.4, and 150 mmol/L NaCl.

The protein was concentrated to 5 mg/ml before further purification by gel filtration (Superdex-200 column, GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.0), 500 mM NaCl, 2 mM DTT, 0.02% (w/v) DDM.

The protein was eluted from the affinity resin by 350 mM imidazole, 25 mM Tris (pH 8.0), 150 mM NaCl, and concentrated to around 10 mg/ml before further purification by gel filtration (Superdex 75, GE Healthcare) in a buffer containing 25 mM Tris (pH 8.0), 150 mM NaCl, 2 mM dithiothreitol.

The released glycans were purified by 80% ethanol precipitation/centrifugation and reduced with sodium borohydride before further purification and desalting.

Before further purification of the RNA with the RNeasy Plant Mini kit (Qiagen), an extra DNAse step (Qiagen) was carried out as recommended by the manufacturer.

Before further purification, 60 µL of mixture was removed and saved as a loading control.

The synthesis was then completed as before to give compounds 48, 55, 60, and 64 in 90 % ee, with further purification by preparative HPLC on a chiral stationary phase providing these compounds in >98 % ee.

Cells were then centrifuged at 700×g for 30 minutes before harvesting of the PMN layer from the 1.095/1.080 interface and further purification by negative magnetic selection using a custom cocktail mix (Stemcell Technologies, Vancouver, Canada) [31].

Double distilled deionized water was used with further purification by Millipore Milli-Q system.

Further purification by silica gel column chromatography afforded the corresponding product in good yield.

Duplex DNA was stored at 4°C prior to further purification by native PAGE.