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Previously published sequences (Clarkson et al., 2004) from four plastid regions (matK, ndhF, trnL-F and trnS-G) were aligned separately using PRANK+F (Löytynoja and Goldman, 2008) and then concatenated to create a combined plastid dataset before further optimization by eye in Mesquite version 2.74 (Maddison and Maddison, 2008).
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Further optimization by incorporating the wild-type T65S mutation in the chromophore improved the quantum yield to 0.87.
Through further optimization by means of concentrating the aliquots, we were able to get further increases in transformation efficiencies.
Unlike other published SSR protocols, this protocol does not require further optimization by the reader, saving considerable time and money.
The reference assembly was subjected to further optimization (designated by '→' in Table 1) by comparative analysis with other Drosophila genomes and gene annotations.
A further optimization is achieved by a reduction factor of 5.02 × 10-4 after preprocessing the overlapped elements before synthesis.
In many such cases further optimization is impaired by the resources required for structural modification of such complex molecular architectures.
Further optimization was achieved by adjusting the foam generation process and varying the composition of the emulsion.
However, animal studies and clinical trials that utilize the technique suggest that it may require further optimization before it can be safely used in humans.
Minor drawbacks such as additional processing time (Figure S2D) may be resolved by further optimization of the code.
Finally, the computational performance of the overall annotation pipeline can be improved by further optimization of the parallel implementations of the individual component tools.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com