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Maybe that's because the '80s represented something of a sports sweet spot, coming after free agency and racial integration had become the norm, but before further expansion, big payroll disparities and salary cap rules began making it tough for top teams to assemble and stay together.
Confluent subcultures were transferred to 24-, 12-, and 6-well plates before further expansion into culture flasks.
The exhaust steam from the HP and IP steam turbines are reheated to 600 °C before further expansion through the IP and LP turbines.
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eBay Now, the same-day shipping service that first debuted for San Francisco residents nearly a year ago before further expansions into San Jose and New York, is preparing to broaden its service yet again, with added support for the Bay Area Peninsula, plus Brooklyn and Queens, as well as Chicago and Dallas later this summer, as previously reported.
Naturally, growth is important but I would argue that it is even more important to erect barriers to entry, or perhaps find a profitable niche and make it your own, before targeting further expansion.
Plan for the mitigation of costs faced in collecting the transfer by households living in remote areas before any further expansion takes place, building on the lessons from Garissa.
It is unlikely that the issue will arise for a decade or two: NATO would be wise to pause before it considers further expansion.
Thereafter, the non-attached cells were removed and the adherent cells were grown to 70% confluency before transfer for further expansion [3].
After 10 days, the cells were harvested, washed and rested for 2 days in 5% HPS culture medium with 5 ng/ml rhIL-15 before analysis or further expansion.
To do this, we need to prepare a cryopreserved allogeneic MSCTRAIL cell bank for further expansion before patient delivery.
All spheroids first enlarged exponentially for a few days and then continued on a linear growth curve for 5 23 weeks before reaching a diameter beyond which there was no further expansion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com