Sentence examples for before further assays from inspiring English sources

Cells were incubated at 37°C for 4 hours before addition of complete medium and cultured for an additional 72 hours before further assays.

Cells were then cultured for the indicated days before further assays.

Therefore, using more than 20% RT products in a qPCR reaction certainly reduces amplification success, as demonstrated by a delayed Cq shift, and thus if cDNA syntheses are to be used for downstream PCRs detection of low copy templates, it would be wise to purify RT reaction products before further assay.

The cells were incubated at 37 °C in the incubator for 48 h before further assay.

The collected serum was stored at –20°C for further assays.

The collected sera were stored at −20°C for further assays.

This is a critical step in validation, providing a check point before investing time and resources on further assay development.

The cells were either used directly for the cytotoxicity assays or cryopreserved before further analysis.

Egg yolk or lyophilized egg powder was first diluted 1 10 in acidified PBS (pH = 5) and centrifuged before further dilutions in PBS and addition to the assay plate.

Complexes were formed by combining stock solutions of salts and chelator to 1000× the desired final assay concentration in DMSO, incubated at 37°C for 30 min before further dilution in complete medium for cell treatment.

Before further processing the roots were removed.