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Finally, we add 1 μL of HCCA matrix solution (α-cyano-4-hydroxycinnamic acid) and leave this to dry before further analysis by MALDI-TOF MS. Each sample is analyzed in triplicate, using the highest score for further analysis.
Firstly, low quality PE reads were removed before further analysis by custom scripts.
Data is anonymised before further analysis by stripping out or coding all information that would allow identification.
The particles were normalized before further analysis by two-dimensional (2D) reference-free alignment and classification using the ML2D algorithm.
To compare STAs across recordings, in which electrode signals may differ in their absolute amplitude values, we z-transform each LFP before further analysis by subtracting its mean and dividing by its standard deviation (each calculated across trials).
For Xi-reactivation assays, MEFs at passage 1 or 2 post-derivation were seeded at a density of 60,000 cells per 12-well well and chemicals in MEF media were added and incubated for 72 h before further analysis (by FISH, immunostaining, or reporter assays).
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The intestinal contents from 3 chickens were pooled by segment before further analysis.
For the profiling study, the expression data were normalized by global median normalization before further analysis.
Before further analysis, each variable was examined for normal distribution by histogram and box plot.
For the remaining 223 miRNAs, raw Ct data were converted to 39 – Ct normalised by global median normalisation before further analysis.
Before further analysis, normal distribution of the variables was tested by applying the Kolmogorov-Smirnov test for the entire study population and each subgroup separately (11).
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