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Following erythrocyte lysis, cells were washed twice again before further analyses.
All PCR amplifications were confirmed by gel electrophoresis before further analyses.
Therefore, any runs of >60 mins of zeros were excluded as 'non worn time' before further analyses.
Because the units of the residualized variables became meaningless, we also z-scored these variables before further analyses.
Outliers, defined as any measurement falling more than 1.5 inner-quartile ranges beyond the nearest inner quartile for a specific tissue, were removed before further analyses.
Cells were allowed to recover in ultra low attachment surface plates overnight in a humidified 37°C incubator before further analyses.
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Before conducting further analyses, statistical model assumptions were examined.
Statistical model assumptions of normal distribution and multicollinearity for variables were examined before conducting further analyses.
Before conducting further analyses, we used jModelTest (http://darwin.uvigo.es/software/jmodeltest.html) to select the best fitting substitution model for our sequences according to the corrected Akaike information criterion.
Transfected cells were maintained in culture for two days before harvesting and further analyses.
It was important, therefore, to first resolve this issue before proceeding with further analyses.
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