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Sections were mounted in 1 : 1 PBS/glycerol solution before examining with a confocal laser scanning microscope.
The medium was removed and cells were washed with PBS, and a new medium containing 5 μM Rh123 was added in the absence and presence of 10 μM 1416 or VER and incubated for 30 min, the cells were then washed twice with ice-cold PBS before examining with the Zeiss 5-LIVE fast CLSM (confocal laser scanning microscopy; Carl Zeiss).
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Before examining genes with a particularly high or low frequency of recombination, we examined properties of the minimum number of recombination events.
These were cultured for 12 h at 25°C before examining the fluorescence with a Zeiss Axiovert 200 M inverted microscope.
Specimens were then mounted on aluminium stubs and sputter coated with gold before examining using scanning electron microscopy (FEI Quanta 200 ESEM, Japan).
Before examining him, I imagined sharing this with him.
Neuronal cultures were then washed again with 1xPBS before being examined with confocal microscopy.
Ultrathin sections were placed on Formvar-coated grids and stained with uranyl acetate and lead citrate before being examined with an electron microscope (FEI Tecnai Spirit, OR, USA).
Some of study participants were referred to a cardiologist, a neurologist, or an otolaryngologist for further evaluation before being examined with our protocol.
Moreover, children were examined carefully in knee to knee position using a dental mirror and their teeth were cleaned and dried by sterile gauze before being examined with respect to ECC and DDE.
Finally, 50 μl pollen suspension were placed onto a glass slide and covered with a glass coverslip before being examined with a UV epifluorescence microscope with a 450 nm excitation filter and a 535 nm emission filter.
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