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Thin sections were stained with uranyl acetate and lead citrate before examination on a 7500 Hitachi transmission electron microscope.
Thin sections (70 nm) were cut and poststained with 4% uranyl acetate and lead citrate in water before examination on a Philips Morgagni 268 D electron microscope (FEI Compagny France, Limeil-Brevannes, France).
The grid was allowed to air dry before examination on a Hitachi 7100 microscope (Hitachi, Germany) fitted with a Gatan Ultrascan 1000 CCD camera (Gatan, Abingdon, UK).
The specimens were critical-point dried using liquid CO2 and sputter-coated with gold before examination on a Stereoscan 240 scanning electron microscope (Cambridge Instr., Cambridge, UK).
Thin sections were stained with uranyl acetate and Reynold's lead citrate before examination on a FEI Tecnai G2 transmission electron microscope operated at 80kVV.
The grid was allowed to air-dry before examination on a Hitachi 7100 transmission electron microscope (Hitachi, Germany) fitted with a Gatan Ultrascan 1000 CCD camera (Gatan, Abingdon, UK) at an operating voltage of 100 kV.
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The grid was dried for 48 h before examination on TEM at an acceleration voltage of 80 kV.
A. thaliana seedlings were incubated with antibodies in suspension and transferred before examination on glass coverslips with a small drop of PBS.
Subsequently, monolayers were washed 4 times with PBS and fixed in 4 % PFA before microscopic examination on an LSM700 inverted confocal microscope (Carl Zeiss, Jena, Germany).
National standards do not require a physician to perform a physical examination on a woman before providing a medication abortion.
They were stored at 4°C and protected from light for ∼24 h before examination and imaging on a fluorescence microscope with a MacProbe v4.3 digital image system.
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