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Thereafter, 1 ml of the contents of each bottle was inoculated on nutrient agar for bacteria and potato dextrose agar for fungi, and incubated appropriately for 48 h before examination for growth.
17 We chose not to withhold β-blocker treatment for more than 1 day before examination, for ethical reasons.
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The parents gave written consent, before the examination, for their children's participation in the study.
Plates were incubated under aerobic conditions for 24 h at 37°C for VRBG and for 48 h at 37°C for MRS before examination.
Saliva samples were collected before and after the MRI examination for cortisol measurements.
All patients had had CVCs inserted before examination and collection of blood for culture.
For testing heat sensitivity, animals were put in plastic boxes and allowed 30 min for habituation before examination.
For testing mechanical sensitivity, animals were put in boxes on an elevated metal mesh floor and allowed 30 min for habituation before examination.
Mice were trained on rotarod for 10 min each day for 3 days before examination.
The grids were then washed in distilled water and stained with 2% ethanolic uranyl acetate for 10 min and with lead citrate for 2 min before examination in a FEI Tecnai 12 TEM [63].
Larvae were raised for at 25°C (4 days) for dauers, or 15°C (5 days) for adults that were subsequently transferred to 25°C for 24 hours before examination.
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