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An MSF spokeswoman confirmed the event to AFP last week, saying it occurred "despite an agreement made between MSF and the joint investigation team that MSF would be given notice before each step of the procedure".
Conversion of DCA was monitored with time on stream and acidity, coke content and chlorine content on the catalyst surface was characterized before each step of the cycle.
All transducers were zeroed to atmospheric pressure before each step of the protocol.
F4ac fimbriae were isolated and purified according to a previously published method [ 10], the only modification being the introduction of filtration of solubilized F4ac fimbriae through 0.20 μm pore size filters (Nylon Membrane 0.2 μm 47 mm PK/100, Millipore Corporation, MA, USA) before each step of precipitation at pH 4 during the purification process to prevent aggregation [ 5].
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My surgeon and I had gone through all of my wishes with each step of discovery before the first cut.
But of course, there's an app for every travel annoyance, and equipping your smartphone before a big trip can make each step of your travels a whole lot less stressful.
In addition, to correct insulin clearance for the contribution of the residual endogenous insulin secretion, we also calculated the metabolic clearance rate of insulin, taking into account the concentrations of C-peptide before and at the end of each step of the clamp, as reported by Ferrannini et al. (15).
Applicants must successfully complete each step of the process before moving onto the next step.
Venter has, in a neat reverse application of the precautionary principle, promoted bioethical debate about each step of his programme well before he carried them out.
In the process, the slides were all rinsed three times in phosphate-buffered saline (PBS) (pH 7.2) before each step, except for the procedure of serum blocking to incubation with the primary antibody.
In the play, Ms. Tasic portrayed an old woman performing the Sisyphean task of climbing a staircase by placing a book under her foot before each step, then returning to a wall of thick, dusty volumes at the bottom of the stairs to get another book before moving to the next step.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com