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Haemodynamics were recorded before each measurement of CO by thermodilution.
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The cuevette was inverted before each measurement to minimize loss of signal due to precipitation of aggregates below the spectrometer beam.
Fish were starved 24 h before each measurement to avoid inclusion of ingested feed in the weight measurements as well as to minimize stress.
In this work, the design and results of applying silver liquid amalgam film modified silver solid amalgam annular band electrode (AgLAF AgSAE), refreshed before each measurement, to voltammetric determination of vitamins C (VC), B1 (VB1) and B2 (VB2) are presented.
Subjects avoided to deep inspiration (DI) before the each measurements of partial and full flow-volume curves.
Before each measurement, the height of the stack of blocks was determined using a ruler, due to small differences in the exact dimensions among gel blocks.
Before each measurement, the stage of the optics module of the spectrometer was cleaned with 100%% ethanol and allowed to dry, and a new background reading was collected.
Quality control included calibration of the gas analyzers before each measurement with the use of known gases of 95% oxygen and 5% carbon dioxide.
Before each measurement, a series of sections was acquired through the vertical axis in order to choose the equatorial section.
Before each measurement, two drops of oxybuprocainhydrochloride combined with sodium fluorescein were instilled for local anesthesia.
Blood pressure was measured three times using an automatic sphygmomanometer after the participants had been resting for 5 min in a sitting position before each measurement; the mean of the three readings was used for analysis.
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