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The test solutions were freshly prepared before each experiment by adding the oil directly to the corrosive solution.
Furthermore, surfaces of titanium and tungsten targets were cleaned before each experiment by a pre-sputtering process for 1 min.
Electrode surface regeneration was performed before each experiment by polishing it with a smooth wet filter paper until a shiny and clean electrode surface was obtained.
H2O2 sample solution (50 mM) was prepared before each experiment by direct dilution of H2O2 (35%; Sigma-Aldrich) in deionized water (DIW) and deaerated by purging it with nitrogen for 20 min. Other chemicals were of analytical grade and used without further purification.
The 1.8 copies/µl standard solution was freshly made before each experiment, by a 10-fold dilution of the standard containing 18 copies/µl.
Calibration of the optical lever was conducted before each experiment by ramping a glass coverslip substrate up and down while in contact with the cantilever.
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To convert this to percentage reduction, fully oxidized and fully reduced cytochrome c absorbance values were measured before the termination of each experiment by addition of ferricyanide (10 μM K3Fe CN 6) and dithionite (10 μl of a 10% solution in 3 ml), respectively.
AβOs were freshly prepared before each experiment and were routinely characterized by size-exclusion chromatography, Western blots using anti-oligomer monoclonal antibody NU4 (Lambert et al, 2007) and, occasionally, by transmission electron microscopy, as previously described (Jurgensen et al, 2011; Sebollela et al, 2012; Figueiredo et al, 2013).
Combined labeling with both 99mTc and ICG-sulfo-OSu for PCSNs was verified by TLC before each experiment (D).
Combined labeling of the PCSN probes with both 99mTc and ICG-sulfo-OSu was verified by TLC before each experiment.
Absence of mtDNA was checked by PCR before each experiment.
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