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Warm up before each exercises.
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Salivary samples were collected before each exercise session to determine estradiol-β-17 and before and after to quantify cortisol.
An identical 5-min warm-up (15 m/min) was performed by both training groups before each exercise session.
Salivary samples were collected before each exercise session to determine estradiol and cortisol concentrations and after each exercise session to determine cortisol concentrations.
[ 23] before each exercise session.
All subjects were checked by a physician before each exercise test.
An identical 5-minute warm-up (15 m/min) was performed by both training groups before each exercise session.
Subjects were instructed to maintain their habitual diet and lifestyle during the 8-week study and to duplicate their diet before each exercise testing/blood collection appointment, as described below.
Before each exercise test, the metabolic analyzer was calibrated with gases of known concentrations (14.01 ± 0.07 % O2, 6.00 ± 0.03 % CO2) and with room air (20.93%O2 and 0.03 % CO2) as per the instruction manual.
To assess the effect of training status on exercise test variables, the number of training sessions that each individual performed during the last 3 weeks before each exercise test was summarized and classified as 1: 0–2 training sessions, 2: 3–5 training sessions and 3: ≥ 6 training sessions.
German patients were tested using a calibrated Monark 834 E cycle ergometer and metabolic cart (CPX/D, MedGraphics, St Paul, MN) while Swiss patients used a Fleisch cycle ergometer and a Quark metabolic cart (Cosmed, Rome, Italy) The metabolic carts were calibrated before each exercise test with two gases of known concentrations.
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