Sentence examples for before each assay from inspiring English sources

Before each assay, animals were acclimated to the experimental conditions for 3 days (once per day).

Fresh solutions were prepared before each assay.

OUR values were measured using a 5331 oxygen probe connected to a 5300A oxygen monitor (YSI, Yellow Springs, OH), which was calibrated before each assay according to the manufacturer's instructions and then was used immediately following calibration to ensure accuracy.

Working solution was prepared freshly before each assay by diluting the stock solution by mixing of stock solution to 50% methanol for an initial absorbance of about 0.700 (± 0.02) at 745 nm, with temperature control set at 30°C.

Fluorescence levels in TetOn-rhTrkB cells were verified before each assay.

Immediately before each assay the tissue was further cut (25 times).

Before starting each assay, animals were kept on electrolyte-free plates (see C. elegans strains section) for at least two generations at 20°C, except gtl-1 tg113 gtl-1 tg113mutant

Plates were allowed to cool for 10 minutes before 30 µl of each assay reagent was added.

At least 10 cell events were acquired for each assay before cell analysis and absolute cell counting.

For endocytosis assay by flow cytometry, RAW264.7 cells were serum-starved for 2 h before each endocytosis assay.

The IgG were quantified using the NanoDrop® ND-1000 spectrophotometer and the required IgG concentration for the parasite growth assay (20 mg/ml) was obtained through appropriate dilution in RPMI 1640 medium before each functional assay.