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Internal standard solution was freshly prepared before each analysis by dilution of a 1 mg/mL isoproterenol stock solution, to a concentration of 100 ng/mL of the compound in perfusion fluid that contains antioxidant (4:1 v/v).
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Before each analysis, the software was externally calibrated by using a protein standard kit (ProteinChip OQ kit; Bio-Rad), and all of the spectra were normalized by means of total ion current.
To compare STAs across recordings, in which electrode signals may differ in their absolute amplitude values, we z-transform each LFP before further analysis by subtracting its mean and dividing by its standard deviation (each calculated across trials).
Lyophilized HHT extract (250 mg) was dissolved in distilled water (25 mL), and then the solution was passed through a 0.2 μm syringe filter (Woongki Science, Seoul, Korea) before analysis by HPLC.> Each calibration curve was established by plotting peak areas versus the concentration of standard solutions.
Additionally, much more parameters are adjustable before performing analysis by using the former two tools.
All the fragments were cloned into TA vector and sequenced before their analysis by EMSA.
Expression data were filtered before pathway analysis by flags.
Firstly, low quality PE reads were removed before further analysis by custom scripts.
The particles were normalized before further analysis by two-dimensional (2D) reference-free alignment and classification using the ML2D algorithm.
Annexin V-FITC and propidium iodide were added to each sample and incubated in the dark for 15 minutes before analysis by a FACS Calibur Flow Cytometer.
When required, denaturation was performed immediately before analysis by heating samples to 45°C for 5 min before placement on ice.
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