Sentence examples for before diluting into from inspiring English sources

Eflornithine was dissolved directly in HMI9-T medium and sterilised by 0.22 μM filtration before diluting into the cell culture.

Protocol 5 4 – PLX4720 must be diluted to 20 mM or less before diluted into media.

Briefly, the CPPs vortexed and then sonicated ENM stock solutions (with the exception of TiO2-NB, which was stirred to prevent mechanical shear) using a water bath sonicator or cup horn sonicator (depending on laboratory availability) immediately before diluting the solutions into complete cell culture media.

Samples were then assayed for concentration before being diluted into sample buffer.

Bacteria to be tested were grown overnight in tryptic soy broth, split 1 10, and incubated to exponential growth before being diluted into each enzyme solution.

For fluorescence pKa measurements, the protein of interest was first dialyzed into dilute buffer (5 mM Tris HCl, pH 7.5) before being diluted into a series of 200 mM phosphate and imidazole buffers at various pH values.

A single colony was used to inoculate a 4 ml culture that was allowed to grow overnight (37°C, 225 rpm) before being diluted into 1 l of LB medium supplemented with ampicillin and l-arabinose.

Antibody-targeted NPs containing 2.5 µg of mRNA/106 cells were mixed into this suspension for an exposure of 2 h at 37 °C before diluting it four-times by adding T cell medium supplemented with 50 IU IL-2/ml.

Tegaserod was dissolved in vehicle (100%1-methyl-2-pyrrolidinone1-methyl-2-pyrrolidinone1-methyl-2-pyrrolidinone1-methyl-2-pyrrolidinonehe final concenthetion ofinalethyl-2-pyrrolidinone was 2.7%.

TPEN was dissolved in DMSO immediately before each use and was diluted into buffer such that buffers contained ≤0.5% DMSO.

Dyes were from Molecular Probes (Eugene, USA) and diluted into water before root staining.