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Experiments calling for sample exposure to inhibitors or reactivating reagents typically involved one or more steps to separate the small molecules from the enzyme before determinations of AChE activity.
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Before determination of scene transitions, the histogram of point distance of each frame is calculated.
The protocol was performed for three separate sessions before determination of the 1-RM to allow familiarization of equipment.
Since the residual human soft tissues partially covered the surface of the implant retrieved from the human body, it was subjected to enzyme treatment before determination of the average surface compositions of the two sides.
In addition, the cost of the enzyme (economics of the process) and quality of the oil are some other factors to consider before determination of the enzyme concentration [40].
Furthermore, when screening for pollutants in soil and leachate at contaminated sites, the results are often required directly, since classification of the soil is needed before determination of remediation techniques (Banar et al. 2007).
The resulting "whey protein-free" casein dispersion (3% casein, 0.02% whey protein) was heated to temperatures between 100°C and 140°C for up to 600 s before determination of renneting properties.
Supernatant was then collected and virus lysed with 0.5% Triton X-100 before determination of p24 levels by ELISA (Ingen).
Liquid media with immerged seedlings were inoculated with S. typhimurium for 48 hours before determination of cfu of the internal bacteria.
This optimization was done in reaction volumes as low as 20 µl before determination of ideal conditions to be used in large-scale reactions.
All strains were pre-grown on glucose before determination of μmax on pentose sugars.
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