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The solution was stirred for 5 min before data were acquired.
Worms were allowed to equilibrate on the slide for 5 min before data were acquired.
After changes to each of the defined positions, 20 minutes were allowed for equilibration before data were acquired.
Subsequently, cCD79a was added and incubated for 30 min at 4°C before data were acquired using FACSAria (BD).
F-fluorodeoxyglucose (5 MBq kg−1; not exceeding 500 MBq) was administered 60 min before data were acquired on a Gemini XL PET/CT scanner (Philips Healthcare, Eindhoven, The Netherlands).
Before data were acquired, we verified that the bilayer was stable for several minutes with a low conductance (<10 pS) across an applied voltage of ±100 mV and that the system capacitance was >110 pF.
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Before laboratory and imaging data were acquired, all patients were classified according to the 1999 ACR NPSLE case definitions [ 2] by one experienced rheumatologist.
To show an influence of cachectic status on lung function tests and muscle and fat tissue in patients before pancreatic surgery, corresponding data were acquired in selected areas using CT scans.
In order to evaluate eventual chromatic changes induced by the treatments, colorimetric data were acquired before and after (40 days and 6 months) application of the consolidant/water-repellent materials.
All resting data were acquired before any task acquisitions.
Data were acquired before and after up to 75% sequential liver resections.
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