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The remaining hexane layer was filtered through a pad of anhydrous sodium sulfate before concentration on a vacuum rotary evaporator at 35 °C.
Note that we confirmed that purified kin401T99N:mGFP was predominantly dimeric before concentration using gel filtration.
Among them, 2 samples had not been detected by the BinaxNOW® Legionella UAC before concentration.
C. rodentium (ATCC 51459) was cultured with aeration in DMEM to log phase (OD650 = 1.0) before concentration by centrifugation.
Sample was stored at -80°C for minimum of 24 hours before concentration determination using a modified Bradford assay [ 13].
Further purification was achieved by size-exclusion chromatography using an S-200 gel-filtration column (GE Healthcare Life Sciences) in the same buffer before concentration and storage.
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As before, increasing concentrations of 17-AAG resulted in a concentration-dependent decrease in PC3 cell viability.
Throughout the experiment, all the urine samples were diluted by 10 times before concentrations were measured.
Following national bans on use of PCBs and DDT in the 1970s, it was 10 15 years before concentrations in lakes and reservoirs decreased by one-half.
Lower risks of death per unit increases in pollutants when concentrations are high may reflect the selective attrition of vulnerable members of the population who die before concentrations reach the maximum level (Wong et al. 2008).
Samples were diluted and filtered before concentrations were determined by non-suppressed anion exchange chromatography (Waters IC-Pak anion exchange column, Waters 430 conductivity detector; Waters, Milford, MA, USA).
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