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To the crude residue was added thiourea (1.9 g, 36 mmol, 1.0eq) dissolved in MeOH (100 ml) and the mixture refluxed for 4 hours before concentrating in vacuo and neutralizing with concentrated ammonium hydroxide solution.
One tenth volume of 2 M L-proline was added before concentrating the protein using 30 kDa cutoff Amicon Ultra concentrators.
Before concentrating on paintings, Hopper (1882-1967) made prints from 1913 to 1923.
She became a producer in 1988, working on the BBC2 comedy Red Dwarf before concentrating on drama.
National governing bodies have been asked by UK Sport to submit their business plans for the next Olympic cycle by February before concentrating wholly on the London Games.
He went on to a series of stunning wins in tournaments at St .Petersburg, Nürnberg, London, and Paris before concentrating on his education.
Before concentrating on drums, the Radiohead founder had written lyrics as a teenager, but when the group started he found himself overshadowed by Yorke's intense style of composition.
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Douglas-Home, as before, concentrated on east-west and Commonwealth matters.
As before, concentrated PAA was then added and infiltrated into the PDMS molds through centrifugation, before drying and finally removal of the completed arrays.
In order to preclude evacuation of TEA during evaporation of the solvents, the amine was trapped in a form of TEA·HCl by acidification of the collected fractions with a slight excess of HCl before concentrated.
The protein fractions were analyzed by SDS-PAGE before pooling, concentrating, and dialyzing (50 mM Tris·HCl, 100 mM NaCl at pH 7.5).
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